By Rodolfo Paoletti; David Kritchevsky
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Additional info for Advances in lipid research. Volume 11
063 gm/ml are formed (Table X). L-~------LT---__, 100 400 500 FIG. 14. Effect of diet on the HDL of a patient with familial LCAT deficiency. Patient M. R. was successively fed a diet containing (A) 35% of the total calories as fat, (B) an isocaloric, fat-free diet, and (C) a diet containing 35% of the total calories as low cholesterol fat. At the end of each dietary period (2, 5, and 4 days respectively) the HDL were prepared by ultracentrifugation and subfractionated by gel filtration on Sephadex G 200.
GLOMSET AND KAARE R. 01 --...... ::::l.. 00 40 60 80 100 120 140 40 60 80 100 120 140 --...... ::::l.. 00 Volume (ml) FIG. 22. Effect of incubation on the HDL of a patient with familial LCAT deficiency. Plasma of patient A. A. 25 grn/ml for 24 hours at 37°C. HDL isolated by preparative ultracentrifugation and subfractionated by gel filtration on Sephadex G 200. 01 M mercaptoethanol. Fron1 Norum et ale (1973). III. 25 gm/ml were used and HDL subfractions from patient A. A. were subsequently isolated by preparative ultracentrifugation and filtration through Sephadex G 200.
HDL of patient M. R. were analyzed by disc gel electrophoresis. Separating gel = 10% polyacrylamide. g respectively. Note that the fast moving component is comparable to the small molecular weight HDL isolated by gel filtration. From Glomset et al. (1973b). 063 gm/ml. In the case of the large and intermediate LDL, changes in unesteri£ed cholesterol occur in the absence of equimolar changes in cholesteryl ester or triglyceride. In the case of the small LDL, major changes in unesterined cholesterol and lecithin occur and a large proportion of the tliglyceride is replaced by cholesteryl ester.
Advances in lipid research. Volume 11 by Rodolfo Paoletti; David Kritchevsky
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