By Robert A. Andersen
Algal Culturing Techniques is a entire reference on all features of the isolation and cultivation of marine and freshwater algae, together with seaweeds. it really is divided into seven elements that hide background, media practise, isolation and purification ideas, mass culturing ideas, cellphone counting and development size strategies, and reports on issues and functions of algal tradition concepts for environmental investigations.
Algal Culturing Techniques used to be constructed to function either a brand new textbook and key reference for phycologists and others learning aquatic structures, aquaculture and environmental sciences. scholars of algal ecology, marine botany, marine phycology, and microbial ecology will benefit from the hands-on technique for culturing various algae from clean and marine waters. Researchers in undefined, comparable to aquaculture, pharmaceutical, foodstuffs, and biotechnology businesses will locate an authoritative and finished reference.
- Sponsored via the Phycological Society of America
- Features colour photos and illustrations throughout
- Describes culturing tools starting from the attempt tube to outdoors ponds and coastal seaweed farms
- Details isolation strategies starting from conventional micropipette to computerized stream cytometeric methods
- Includes purification, progress, upkeep, and cryopreservation techniques
- Highlights tools for estimating algal populations, progress charges, keeping apart and measuring algal pigments, and detecting and culturing algal viruses
- Features a entire appendix of approximately 50 algal tradition medium recipes
- Includes a thesaurus of phycological terms
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Extra info for Algal Culturing Techniques
Medium K (Keller et al. 1987) was developed from culturing fastidious oceanic phytoplankton, and it has been tested on 200 ultraplankton clones representing seven algal classes. It is not recommended for coastal species. This medium includes selenium, both nitrate and ammonium, increased chelation, reduced copper, and a moderate level of pH buffering. The chelation to total metal ratio is 10 : 1, and the EDTA concentration is 10-4 M. There is also a synthetic counterpart. The recently formulated MNK medium and Pro99 medium (see later discussion and Appendix A) are also formulated for open ocean phytoplankton, and preliminary results suggest that they are superior to K medium for certain algae (coccolithophores and Prochlorococcus, respectively).
Vitamins Usually three vitamins—vitamin B12 (cyanocobalamin), thiamine, and biotin—are added, but very few algae need all three vitamins (Provasoli and Carlucci 1974). The general order of vitamin requirements for algae is vitamin B12 > thiamine > biotin. If large-scale culturing of a single species is the goal, check what vitamins this species requires. It may be possible to omit the addition of two of the three vitamins, because most species require only one or two. 2-mm ﬁlter) after the medium has been autoclaved.
Phycol. 16:28–35. Harrison, P. , Yu, P. , Thompson, P. , Price, N. , and Phillips, D. J. 1988. Survey of selenium requirements in marine phytoplankton. Mar. Ecol. Prog. Ser. 47:89–96. Keller, M. , Bellows, W. , and Guillard, R. R. L. 1988. Microwave treatment for sterilization of phytoplankton culture media. J. Exp. Mar. Biol. Ecol. 117:279–83. Keller, M. , Selvin, R. , and Guillard, R. R. L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol. 23:633–8. , and Pytkowicz, R. 1967.
Algal Culturing Techniques by Robert A. Andersen
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